Cloning and Expression Analysis of HbHEV3 Gene from Hevea brasiliensis

Based on EST sequence,we cloned a gene named HbHEV3 coding prohevein.Sequence analysis revealed that HbHEV3 ORF cDNA length was 630 bp,encoding 209 amino acids.HbHEV3 shared high identities to prohevein from Hevea brasiliensis and other plants,had the typical structure of prohevein,containing a signal peptide,a conserved hevein/chitin binding domain and a Barwinn domain.The 1 050 bp promoter sequence of HbHEV3 was isolated and several regulatory elements related to hormone and stress responses were found in promoter region.The real-time PCR results indicated HbHEV3 was expressed in all detected organs,in which,the transcripts of HbHEV3 were higher in latex than that in other organs,and HbHEV3 was also induced significantly by ethephon.The results showed that HbHEV3 may be involved in ethylene-mediated defense responses and may play an important role in the process of latex aggregation.