Clone and Expression of HbNAC24 Gene from Hevea brasiliensis

The whole open reading frame(ORF)sequence of HbNAC24 was cloned from cDNA of Hevea brasiliensis latex,and the transcriptional activation activity and expression profile of HbNAC24 were carried out using Real-time PCR and yeast assay system.The results indicated that:(1)HbNAC24 has an OPR of 1167 bp,encoding aprotein with 388 amino acids.It's N-terminal region had a conserved NAC domain from amino acid 7 to 174.(2)Yeast assay showed that HbNAC24 protein possessed transcriptional activation activity and the activation domain was located in the highly divergent C-terminal region in yeast assay system.(3)Sequence alignment and phylogenetic analysis revealed that HbNAC24 was a member of a NAC transcription factor family belonging to TIP subgroup.(1)The real-time PCR indicated that expression level of HbNAC24 in leaves was significantly higher than that in other parts of the rubber tree.The expression of HbNAC24 in latex was induced by tapping and jasmonic acid(JA).The results suggested that HbNAC24 may be involved in response to stress and developmental processes in rubber tree.