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Cloning and Expression Analysis of HbNAC1 in Hevea brasiliensi

Zhili Zhang

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Abstract

Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis,a full-length cDNA,named as HbNAC1,was cloned from H.brasiliensis by RACE-PCR,encoding a NAC transcription factor.The full-length of HbNAC1 cDNA was 1419 bp with an open reading frame(ORF) of 930 bp,and encoding 309 amino acids.The deduced HbNAC1 with NAM domains had the typical characteristics of NAC family,and homologous to NACs in Populus trichocarpa,Ricinus communis,Oryza sativa and Arabidopsis thaliana of 79%,80%,57% and 60%,respectively.Phylogenetic analysis showed that HbNAC1 was classified into the Ⅱ subfamily of NAC family.Semi-quantitative RT-PCR analysis indicated that the expression of HbNAC1 was rich in latex,and significantly enhanced by ethephon stimulation.

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What this paper is about

Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis,a full-length cDNA,named as HbNAC1,was cloned from H.brasiliensis by RACE-PCR,encoding a NAC transcription factor.The full-length of HbNAC1 cDNA was 1419 bp with an open reading frame(ORF) of 930 bp,and encoding 309 amino acids.The deduced HbNAC1 with NAM domains had the typical characteristics of NAC family,and homologous to NACs in Populus trichocarpa,Ricinus communis,Oryza sativa and Arabidopsis thaliana of 79%,80%,57% and 60%,respectively.Phylogenetic analysis showed that HbNAC1 was classified into the Ⅱ subfamily of NAC family.Semi-quantitative RT-PCR analysis indicated that the expression of HbNAC1 was rich in latex,and significantly enhanced by ethephon stimulation.

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Available abstract

Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis,a full-length cDNA,named as HbNAC1,was cloned from H.brasiliensis by RACE-PCR,encoding a NAC transcription factor.The full-length of HbNAC1 cDNA was 1419 bp with an open reading frame(ORF) of 930 bp,and encoding 309 amino acids.The deduced HbNAC1 with NAM domains had the typical characteristics of NAC family,and homologous to NACs in Populus trichocarpa,Ricinus communis,Oryza sativa and Arabidopsis thaliana of 79%,80%,57% and 60%,respectively.Phylogenetic analysis showed that HbNAC1 was classified into the Ⅱ subfamily of NAC family.Semi-quantitative RT-PCR analysis indicated that the expression of HbNAC1 was rich in latex,and significantly enhanced by ethephon stimulation.

Key concepts: Hevea brasiliensis, Complementary DNA, Ethephon, Biology, Ricinus, Subfamily, cDNA library, Open reading frame

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