Effect of octreotide preconditioning on brain injury after ischemia-reperfusion in the rabbit liver

[Objective] To research the effect of octreotide on the brain injury after ischemia-reperfusion in rabbit liver.[Methods] Pring's maneuver rabbit hepatic ischemia-reperfusion models were established.24 adult New Zealand rabbits were randomly divided into equal three groups:groupⅠ(sham operative group),group Ⅱ(ischemia-reperfusion) and group Ⅲ(octreotide preconditioning group).To group Ⅲ,we injected octreotide of 20 μg/kg to abdominal cavity and octreotide of 30 μg/kg to skin following,while taking octreotide to 2 mL with 0.9% physiological saline.To groupⅠand group Ⅱ,we replaced octreotide with equal physiological saline.30 min later,the changes of MAP,HR in every group were recorded at the time before ischemia(T1),30 min(T2) after ischemia,30 min(T3),60 min(T4),120 min(T5),240 min(T6) after reperfusion.These rabbits were killed after 240 min after reperfusion.Then the changes of pathomorphology were observed under light microscope and ultrastructure in the brain cell by electromicroscope and detected the apoptosis of their brain organizes by TUNEL.The contents of superoxide dismutase(SOD) and malondialdehyde(MDA) in brain tissue of each group 240 min after reperfusion were measured.[Results] ① The MAP,HR of group Ⅱ and group Ⅲ were lower than that of group Ⅰat T2,moreover,group Ⅱ were lower than that of group Ⅲ at T2 to T4(P 0.05,P 0.01).② The changes of pathomorphology and ultrastructure of the brain in group Ⅲ was slighter than in group Ⅱ.③ The apoptosis of the brain organizes by TUNEL under 5 light microscopes,and the apoptosis counts of group Ⅱ and group Ⅲ were more than that of group Ⅰ,and group Ⅲ were less than that of group Ⅱ(P 0.05).④ The levels of MDA in group Ⅲ were lower than that in group Ⅱ(P 0.05).The levels of SOD in group Ⅲ were higher than that in group Ⅱ(P 0.05).[Conclusion] Octreotide can reduce hemodynamic severe fluctuation,relieve significantly the injury of the brain's pathomorphology and ultrastructure,restrain apoptosis,reduce release of oxygen free radical.Octreotide preconditioning can relieve brain injury after hepatic ischemia-reperfusion in rabbit.