Study on the Immune Function of Dendritic Cells from Peripheral Blood of Patients with Positive preS1 Protein Chronic Hepatitis B

Objective To study the immune function of dendritic cells(DC)from peripheral blood of patients with positive preS1 protein chronic hepatitis B(CHB).Methods Monocytes were purified from peripheral blood of health volunteers(control group1,10 cases),patients with negative preS1 protein chronic hepatitis B(control group2,10 cases)and patients with positive preS1 protein chronic hepatitis B(experimental group,10 cases)with incubation of granulocyte/macrophage colony stimulating factor(GM-CSF),interleukin-4(IL-4)and interferon-γ(IFN-γ)which was added on day 6.The abilities to stimulate the proliferation of allogenic T cells were evaluated by MTT assay,and the levels of TNF-α and IL-12 p40+p70 in culture supernatant of DCs were detected by ELISA.Results The abilities to stimulate the proliferation of allogenic T cells by DCs and the levels of TNF-α and IL-12 p40+p70 in culture supernatant of DCs in experimental group were significantly less than those in control group 1(P0.01),but there was no difference versus control group2(P0.05).Conclusion It is suggested that the patients with CHB have the defective immune function of DCs.In the period of HBV persistence infection,there is no direct relativity between the function of DC from peripheral blood of CHB and positive preS1 protein.