THE EFFECTS OF SODIUM SELENITE ON THE FUNCTION OF DENDRITIC CELLS FROM PERIPHERAL BLOOD OF CHRONIC HEPATITIS PATIENTS

Objective To explore the methods of improving the immune function of dendritic cells(DCs) from patients with chronic type B hepatitis in vitro. Methods Monocytes from type B hepatitis patients and healthy controls were incubated with GMCSF and IL-4 and IFN-α to induce dendritic cells(DCs) generation.Then the nutrien-concentration sodium selenite was added for co-culture before the maturity of DCs.The samples were separated into three groups: hepatitis-B patients (group A),hepatitis-B patients with Se(group B),healthy control(group C).The phenotypes of DCs including CD80,CD86 were characterized by flow cytometry(FCM) and the response of allogeneic lymphocytes was detected by MTT assay.The concentration of cytokines in the supernatant such as IL-12 was tested by ELISA assay. Results After the nutrien-concentration Se was added for co-culture,the proliferation ability of isotype xenoma lymphocyte stimulated by DCs was obviously streng thened compared with group A,and the excretion level of IL-12 also increased(P0.01).There was no significant difference between group B and group C.Conclusion Co-cultured with the nutrient-concentration Se in vitro,DCs could stimulate lymphocyte proliferation reaction effectively,improve the excretion of IL-12,and recover DC's immunological function partly,which may provide a theory for future immune therapy by DCs for chronic type B hepatitis patients.