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2011•Xi'nan nongye xuebaoRequires access

Callus Induction and Plant Regeneration of Saffron

Yang-Dong Guo

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Abstract

The corms of saffron were used as explants to establish a regeneration protocol for Crocus sativus L.The corms incubated in MS+0.5 mg/L BA +1.0 mg/L NAA+7 %CM at first,then transferred to MS +5 mg/ L BA +5 mg/ L NAA for callus induction.Callus formation was observed after 51 days,culture and the callus induction rate was 65 %.After four weeks callus induction,the shoot regeneration rate(95 %) was obtained when 2 mg/L BA was combined with 1 mg/L NAA.The shoot buds were fragmented and transferred to root induction medium containing 0.5 mg/L IBA.Plantlets were then successfully transplanted to greenhouse.

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What this paper is about

The corms of saffron were used as explants to establish a regeneration protocol for Crocus sativus L.The corms incubated in MS+0.5 mg/L BA +1.0 mg/L NAA+7 %CM at first,then transferred to MS +5 mg/ L BA +5 mg/ L NAA for callus induction.Callus formation was observed after 51 days,culture and the callus induction rate was 65 %.After four weeks callus induction,the shoot regeneration rate(95 %) was obtained when 2 mg/L BA was combined with 1 mg/L NAA.The shoot buds were fragmented and transferred to root induction medium containing 0.5 mg/L IBA.Plantlets were then successfully transplanted to greenhouse.

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Available abstract

The corms of saffron were used as explants to establish a regeneration protocol for Crocus sativus L.The corms incubated in MS+0.5 mg/L BA +1.0 mg/L NAA+7 %CM at first,then transferred to MS +5 mg/ L BA +5 mg/ L NAA for callus induction.Callus formation was observed after 51 days,culture and the callus induction rate was 65 %.After four weeks callus induction,the shoot regeneration rate(95 %) was obtained when 2 mg/L BA was combined with 1 mg/L NAA.The shoot buds were fragmented and transferred to root induction medium containing 0.5 mg/L IBA.Plantlets were then successfully transplanted to greenhouse.

Key concepts: Callus, Corm, Explant culture, Crocus sativus, Shoot, Botany, Regeneration (biology), Tissue culture

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