2007•Guocheng gongcheng xuebaoRequires access
Induction and Regulation of Crocus sativus L. Embryogenic Callus
Chen Shu-an, Wang Xiao-dong, Bing Zhao, Yuchun Wang
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Abstract
The induction and regulation of embryogenic callus were investigated to promote the growth and shoot induction rate for micropropagation of Crocus sativus L. The embryogenic callus line was obtained easily from one corm callus line (corm 1). After 25 d, the maximum proliferation rate of callus reached 8.9 g/g under the optimized condition for cell growth when the callus was cultured on B5 medium supplemented with 400 mg/L CH, 0.25 mg/L NAA and 3.0 mg/L 6-BA at 22 ℃ in darkness. After 45 d, the maximum shoot induction rate of callus reached 45% under the optimized condition for shoot induction when the callus was cultured on 1/2 B5 medium supplemented with 400 mg/L CH, 0.25 mg/L NAA and 3.0 mg/L 6-BA at 22 ℃ under 16 h light with the intensity of 31.74 μmol/(m2s) per day, while the highest shoot induction rate of Crocus sativus L. callus was reported only about 20% abroad.