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EFFECTS OF iNOS-DERIVED NO ON APOPTOSIS FOLLOWING FOCAL CEREBRAL ISCHEMIA AND REPERFUSION IN RATS

Zhao Yu

Abstract

ObjectiveTo investigate the effects of iNOS-derived NO on apoptosis following focal cerebral ischemia and reperfusion in rats. MethodsFocal cerebral ischemic model was induced by the occlusion of left middle cerebral artery. Aminoguandine, a selective inhibitor of iNOS, was given intraperitoneally. The rats were sacrificed at 48 h of reperfusion after 2 h of ischemia. The content of NO was measured by nitriate reductase method.The expression of NT was examined by flow cytometry.The cellular apoptosis was examined by flow cytometry and TUNEL. ResultsThe number of TUNEL positive cells and the apoptotic percentage were decreased markedly in Aminoguandine groups. ConclusioniNOS-derived NO may play a role in apoptosis following focal cerebral ischemia and reperfusion.

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Key concepts: TUNEL assay, Apoptosis, Ischemia, Flow cytometry, Middle cerebral artery, Medicine, Reperfusion injury, Occlusion