Experimantal Study on Focal Cerebral Ischemia and Reperfusion Injury

Objective To observe brain histopathological changes at different time points after focal cerebral ischemia and reperfusion,and the relation between them and the nerve function score at the corresponding time.Methods To make use of the focal cerebral ischemia and reperfusion model induced by a suture method which preferred by Zea Longa in rats,55 adult Wistar rats randomly allocated to the ischemia group,sham-operation group,ischemia and reperfusion group,five rats in each group.HE staining and TUNEL method were used to investigate the neuron apoptosis and test neuronal apoptosis histopathology by electron microscope after cerebral ischemia and reperfusion.Results Neuronal function deficit was most serious in 0～6 h after reperfusion,after that the function became better,while being exacerbated after 24 h.TUNEL assay showed that within 48 h of refperfusion,cellular AI in the cortex increased with prolong of the reperfusion time.Conclusions Focal cerebral ischemia and reperfusion model induced by a suture method in rats is successfully used in this trial to study the mechanism of cellular damage and cerebral protection after ischemia and reperfusion.The neuronal death after ischemia is via apoptosis and necrosis.Moderate to severe ischemia mainly results in necrosis,while mild ischemia mainly results in apoptosis.