Cell apoptosis and its relation to the expression of Caspase-3 protein after focal cerebral ischemia-reperfusion in rats

Aim:To study the cell apoptosis and its molecular mechanism for focal cerebral ischemia reperfusion model rats.Methods:The model of focal cerebral ischemia reperfusion was established with the suture occluded method.Caspase 3 protein in rats at different time polits of cerebral ischemia reperfusion was observed by immunohistochemical method,the cell structure was examined under light microscope,and the content of MDA was measured. Results:There was no significant cell necrosis in 1 h ischemia reperfusion group.There was significant cell necrosis and apoptosis in 2 h, 3 h,and 6 h ischemia reperfusion groups, but there was no injury in sham operation group. The level of Caspase 3 protein in 6 h ischemia reperfusion group was significantly higher than those of other groups. The time phase pattern of Caspase 3 protein was consistent with that of cell apoptosis. The content of MDA was significantly higher in 2 h ischemia reperfusion group than in that of 0.5 h ischemia reperfusion groups. Conclusion:The injury of neuronal cell aggravates with the ischemia reperfusion time;treatment within 1 h after ischemia is effective,and the increase of Caspase protein would be one of the molecular mechanisms of cell apoptosis in ischemia reperfusion.