Effect of apoptosis and DNA damage on human umbilical vein endothelial cells induced by citreoviridin

Objective To investigate the effects of apoptosis and DNA damage on human umbilical vein endothelial cells(HUVEC) caused by citreoviridin.Methods HUVECs were isolated from the neonatal umbilical core in 1 hour(with informed consent form of the family) and cultured.The injury models were established by dealing with CIT at different concentrations of 1 μmol/L,2 μmol/L,5 μmol/L and 10 μmol/L for 48 hours.Cells′ morphous was observed under light microscope during the period of growth.Levels of cells′ viability were assessed by MTT assay.Flow cytometry was used to demonstrate apoptotic changes.Single cell gel electrophoresis(SCGE) was used to measure the DNA damage of the cells.Results Cells in control grew better than CIT groups,Some of the cells in CIT groups were round.The level of cells′ viability in control was higher than those of CIT groups at doses of 2 μmol/L,5 μmol/L and 10μmol/L(P0.05).Apoptotic rates in CIT groups of 2 μmol/L,5 μmol/L and 10 μmol/L were higher than that of the control.The levels of damaged DNA in CIT groups of 5 μmol/L and 10 μmol/L were significantly diffenent from that of the control.Conclusion CIT causes down-regulation of the viability of HUVEC,induces apoptosis of HUVEC and results in DNA damage.