Endothelial cell injury induced by different concentration fluoride in vitro

Objective: To observe the lesion of human umbilical vein endothelial cells(HUVECs) induced by different doses of fluoride.Methods: HUVECs were cultured with fluoride in various concentration(120,240,360,480,600,720,840,960?μmol/L),24 hours later,the morphologic changes were observed,the cell activity was measured by MTT method,and the MDA,NO and SOD were determined.Results: The cell increased when treated with fluoride of 120?μmol/L to 240?μmol/L,whereas decreased with fluoride of 600?μmol/L to 960?μmol/L,and the cells were injured.The cell activity of the group of 120?μmol/L and 240?μmol/L significantly enhanced compared with the normal control,but that of the group of over 600?μmol/L markedly lowered compared with the normal control.NO and SOD decreased,but MDA increased in the group of over 240?μmol/L.Conclusions: Fluoride of low concentration can promote the cell activity,but that of high concentration can injure the cell,and the higher the concentration is,the heavier the injury is.