4-hydroxynonenal induces the apoptosis of bronchial epithelial cells

Objective To explore the role of 4-hydroxynonenal(4-HNE)in bronchial epithelial cells(16-HBE)apoptosis.Methods 16-HBE cells were divided into four groups:control,10 μmol/L,30 μmol/L,50 μmol/L 4-HNE stimulation groups.16-HBE were stimulated by 10 μmol/L,30 μmol/L,50 μmol/L 4-HNE for 4 hours,and the apoptosis of cells were observed.The phosphorylation of JNK and caspase-3 were determined by Western blot.Results Cell apoptosis was obviously found in 30,50 μmol/L 4-HNE stimulating groups by Giemsa staining.The numbers of apoptosis in control,10 μmol/L,30 μmol/L,50 μmol/L 4-HNE stimulation groups were 1.94±1.03,2.03±1.04,43.36±1.3,and 65.92±3.45,respectively.There were significant differences of 30 and 50 μmol/L 4-HNE to control and 10 μmol/L 4-HNE groups(P0.01).The expression of phosph-JNK in four groups was not different,but the csapase-3 expression was significantly higher in 30,50 μmol/L 4-HNE groups compared to the control,10 μmol/L 4-HNE groups.Conclusion 4-HNE induces apoptosis of bronchial epithelial cells,which may be related to activation of caspase-3.