2010•Chinese Journal of Health Laboratory TechnologyRequires access
Synthesis and identification of artificial antigens of ciprofloxacin
DU Shu-lian
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Abstract
Objective:To synthesize and identify artificial antigen of ciprofloxacin.Methods:The immunogen CIP-BSA(ciprofloxacin-bovine serum albumin) were synthesized by the method of N-Hydroxysuccinimide active ester.These artificial antigens were identified by UV absorption method and the animal immunization test.Results:The coupling molar ratios of the hapten and carrier proteins were 6 for CIP-BSA.The antibodies showed the 50% inhibition of control values(IC50) of 38 ng/ml by ci-ELISA.Conclusion:These artificial antigens were successfully synthesized and the anti-ciprofloxacin antibody received,which provides the experimental basis for the further study of ELISA or TRFIA.