Synthesis and identification of enrofloxacin antigen.

The immunogen ENR-BSA(enrofloxacin-bovine serum albumin) were synthesized by the N-Hydroxy-succinimide active ester method.The conjugations were identified by SDS-PAGE,UV and animal immunization test.The coupling molar ratios of the hapten and carrier proteins were 5:1 for ENR-BSA.The antibodies showed the 50% inhibition of control values(IC50) of 60 ng/mL by ci-ELISA.The antigenic conjugates were artificially synthesized and the anti-enrofloxacin antibody received,which provides the experimental basis for the further study of ELISA or TRFIA.