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2013•Advanced materials researchOpen access

The Synthesis and Identification of a Ciprofloxacin Artificial Antigen

Long Xu, Li Xin Zhu, Ren Rong Liu, Yan Fan, Wei Meng

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Abstract

The artificial antigen ciprofloxacin-bovine serum albumin (CPFX-BSA) and ciprofloxacin-ovalbumin (CPFX-OVA) were synthesized by carbodiimide (EDC) method and sodium periodate oxidation method, respectively. These two kinds of antigens were identified by the UV absorption method and the animal immunization test, which showed that they were synthesized successfully. The successful synthesis of ciprofloxacin artificial antigen is important to establish immunoassay of ciprofloxacin.

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What this paper is about

The artificial antigen ciprofloxacin-bovine serum albumin (CPFX-BSA) and ciprofloxacin-ovalbumin (CPFX-OVA) were synthesized by carbodiimide (EDC) method and sodium periodate oxidation method, respectively. These two kinds of antigens were identified by the UV absorption method and the animal immunization test, which showed that they were synthesized successfully. The successful synthesis of ciprofloxacin artificial antigen is important to establish immunoassay of ciprofloxacin.

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Available abstract

The artificial antigen ciprofloxacin-bovine serum albumin (CPFX-BSA) and ciprofloxacin-ovalbumin (CPFX-OVA) were synthesized by carbodiimide (EDC) method and sodium periodate oxidation method, respectively. These two kinds of antigens were identified by the UV absorption method and the animal immunization test, which showed that they were synthesized successfully. The successful synthesis of ciprofloxacin artificial antigen is important to establish immunoassay of ciprofloxacin.

Key concepts: Ciprofloxacin, Carbodiimide, Ovalbumin, Antigen, Bovine serum albumin, Immunoassay, Immunization, Chemistry

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