2014•China Animal Husbandry & Veterinary MedicineRequires access
Expression of Goose Parvovirus VP3 Gene in Baculovirus Expression System
Zhiqian Wang
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Abstract
The fusion VP3 gene sequence of goose parvovirus(GPV)was amplified by PCR using apair of specific primers from GPV FY89 strain.The PCR product was cloned into pFastBac/HBM-TOPO vector and transformed into DH10 Bac cells.Recombinant Bacmid-VP3 was transfected into Sf9 insect cells,and a fusion protein about 61 ku was expressed and identified by SDS-PAGE analysis.The antigenicity of the recombinant VP3 protein was confirmed by Western blotting.