2007•Zhongguo shouyi kexueRequires access
Eukaryotic expression of NS2 gene and nonoverlapping sequence of VP1 gene to VP3 gene of goose parvovirus and detection of the expressed proteins
Junwei Wang
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Abstract
The pBlueBacHis2A insect expression system was chosen to screen the effectual detecting antigens against goose parvovirus(GPV).Recombinant plasmids pBlueBacHis2A-GPV-NS2 with NS2 gene of GPV and pBlueBacHis2A-GPV-VP(1-3) with the nonoverlapping sequence of VP1 gene to VP3 gene of GPV were constructed by PCR and double restriction enzymes.Then,the two plasmids were transfected into sf9 cells to obtain recombinant viruses.The expressed proteins were 54 ku and 24 ku in size from the two recombinant plasmids,respectively,and their specificities were detected by Western-blotting,Dot-ELISA and indirect immunofluence assay.All the results confirmed that the recombinant proteins which were expressed successfully in sf9 cells had good specificity.