Eukaryotic Expression of VP3 Gene of Goose Parvovirus YBLJ Strain

To investigate the biological characteristics of YBLJ VP3 gene of goose parvovirus(GPV),according to the cloned gene sequence of GPV YBLJ strain in GenBank(JN836326),a pair of specific primers with Hind Ⅲ and XhoⅠ sites were designed.VP3 gene was amplified by PCR,and subcloned into pcDNA 3.1.The recombinant plasmid was transferred into Vero cells with Lipofectamine 2000,and its expression was detected by RT-PCR and indirect immunofluorescent assay(IFA).The results showed that the eukaryotic expression vector of pcDNA-VP3 was successfully constructed,a 1 605 bp specific band appeared in the experimental group by RT-PCR,the expression of VP3 was detected by IFA,which laid a foundation for development of GPV nucleic acid vaccine.