Studies on Pharmacokinetics and the Elimination Regularity of Chloramphenicol Residues in Paralichthys olivaceus (T&S) in Vitro

The pharmacokinetics and elimination regularity of CAP in Paralichthys olivaceus (TS) were firstly studied. The concentrations of CAP were determined by High Performance Liquid Chromatography, and the pharmacokinetic data derived from the experiment were analyzed by non-compartmental methods based on statistical moment theory. The results indicated that; 1) The concentration-time curves of CAP showed an obvious double-peak phenomenon after oral administration at a dose of 80 mg · kg-1; The main pharmacokinetic parameters were as follows; Tmax was about 2 h, Cmax(1) was 15. 01 , 11. 80 , 10. 35 , 8. 56, 5. 21 μg · mL-1 in gill, liver, kidney, blood, muscle respectively, Cmax(2) was smaller than the corresponding Cmax(1),Tmax(2) was 8 h, AUC was 176. 87 , 133. 77 , 118. 77 , 65. 33 , 50. 36 mg · h7L-1 in kidney, gill, liver, blood, muscle respectively, t1/2 4. 89 - 10. 39 h, MRT 8. 67 - 17. 05 h, This showed that the absorption was rapid after oral administration of CAP in paralichthys olivaceus (TS), but the residence time was long. 2) T1/2 were 39. 4 - 115. 5 h after oral administration at dose of 40 mg · kg-1 for five consecutive days, which showed that the elimination of CAP from the body was retarded and the residual amount in the body was great. Furthermore, residue concentrations in the kidney and liver were the highest.