The Effect of Ulinastatin on the Liver Function after Limb Ischemia-reperfusion in Rats

Objective To observe the protective effect of ulinastatin on the liver function after limb ischemia-reperfusion in rats and the potential mechanism.Methods The developments of healthy SD rat hind limb ischemia-reperfusion model,24 healthy SD male rats were randomly divided into 3 groups of 8.Detect the liver function,concentration of serum tumor necrosis factorα(TNF-α),interleukin(IL)-10 factor,electron microscopy of liver mitochondrial structure and optical microscopy structure of the liver Pathology.Results Liver function parameters were measured,the level of ALT,AST,LDH increased in the control group,experimental group than that in sham-operated group,and the level of ALT,AST,LDH increased in the experimental group than that in the control group.The level of inflammatory cytokines such as TNF-α,IL-10 was increased in the control group,experimental group than that in sham-operated group.The level of TNF-αdecreased in experimental group than that in the control group.The level of IL-10 increased in control group,experimental group than that in sham-operated group,the level of IL-10 increased in experimental group than that in sham-operated group.There were significant difference all of the above(P0.05).The structure of mitochondria:the sham-operated group was 0 grade,the control group was 2～3 grade,and the experimental group was 1 grade.Conclusion Ulinastatin has protective effects on the liver function after limb ischemia-reperfusion in rats.The theory was to decrease the blood serum of TNF-α and raise the blood serum of IL-10,so the inflammatory reaction is inhibited to protect the herpetic function and the ultra structures of mitochondria.