Determination on the Content of Total Flavonoids in Different Species of Camellia chrysantha(Hu)Tuyama.
Wei Xiu-zhi
Abstract
Wei Xiu-zhi
Abstract
[Objective] The aim was to establish the determination methods for the content of total flavonoids in different species of C.chrysantha.[Methods] Total flavonoids were extracted from C.chrysantha using ethanol water bath backflow,then,the content of total flavonoids was determined using UV-VIS.[Result] The regression equation is Y=13.65X-0.004 5,it indicates that within the range of 8.64-69.12 μg/ml,the linear relation is better(R=0.999 9),average recovery ratio is 100.40% and RSD is 1.50%.[Conclusion] The method is simple and reliable,can be used to control the quality of different species of C.chrysantha;the contents of total flavonoids in different species of C.chrysantha are different.
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[Objective] The aim was to establish the determination methods for the content of total flavonoids in different species of C.chrysantha.[Methods] Total flavonoids were extracted from C.chrysantha using ethanol water bath backflow,then,the content of total flavonoids was determined using UV-VIS.[Result] The regression equation is Y=13.65X-0.004 5,it indicates that within the range of 8.64-69.12 μg/ml,the linear relation is better(R=0.999 9),average recovery ratio is 100.40% and RSD is 1.50%.[Conclusion] The method is simple and reliable,can be used to control the quality of different species of C.chrysantha;the contents of total flavonoids in different species of C.chrysantha are different.
Key concepts: Chemistry, Linear relationship, Linear regression, Mathematics, Food science, Statistics