The establishment of detecting method for porcine reproductive and respiratory syndrome virus(PRRSV)

Based on highly conservative ORF6 gene sequence of PRRSV on GeneBank,a pair of primers was designed and synthesized.The RT-PCR method for detecting PRRSV was established.PRRSV-QD RNA was extracted from virus-infected Marc-145 cell and then amplified by RT-PCR.The positive 266bp specific fragment was obtained as expected.Under the same conditions,however,the results for detection of PRV,PPV,PCV and Marc-145 cell by RT-PCR were all negative.The amplified sequence of PCR product of PRRSV-QD shares 94.4%-97.4% homology with the published sequence of other PRRSV strains from GeneBank.The results indicate that RT-PCR method in this research has high sensitivity and specificity,and could be further applied for PRRSV clinical diagnosis.