Studies on Detection of Porcine reproductive and respiratory syndrome virus in Tissue Sample by Reverse Transcription Polymerase Chain Reaction

According to the published sequence of PRRSV genome, a pair of specific primers (N1/N2) was designed based on the gene encoding the N protein of PRRSV. The region between the primers was about 380 bp. A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of PRRSV in clinical lung tissue samples. Five clinical lung samples were detected by RT-PCR and all were identified as PRRSV positive, two of them were further cloned and sequenced. The result showed that both genes are 372 bp in length and shared very high homology to the ORF7 gene of PRRSV VR-2332 strain.In conclusion, the RT-PCR is specific and rapid and can be applied to diagnose PRRS clinically.