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2012•Chinese Journal of Spectroscopy LaboratoryRequires access

Determination of Lactic in Rat Serum by RP-HPLC

Dong Xiu

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Abstract

The method for determination of lactic in rat serum was developed by reversed-phase high performance liquid chromatography(RP-HPLC) with ultraviolet detector.Protein in serum samples was precipitated by perchloric acid solution,and Eclipse plus C18 column was used with 5% methanol-water solution containing 0.2% phosphoric acid as the mobile phase at a flow rate of 0.8mL·min-1,and detection wavelength was 210nm,quantified by external standard method.Lactic had good linear in the range of 4.88×10-3—5g·L-1(r=0.9999),and the detection limit was 1.5×10-3g·L-1.The method is fast,sensitive with good precision.

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What this paper is about

The method for determination of lactic in rat serum was developed by reversed-phase high performance liquid chromatography(RP-HPLC) with ultraviolet detector.Protein in serum samples was precipitated by perchloric acid solution,and Eclipse plus C18 column was used with 5% methanol-water solution containing 0.2% phosphoric acid as the mobile phase at a flow rate of 0.8mL·min-1,and detection wavelength was 210nm,quantified by external standard method.Lactic had good linear in the range of 4.88×10-3—5g·L-1(r=0.9999),and the detection limit was 1.5×10-3g·L-1.The method is fast,sensitive with good precision.

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Available abstract

The method for determination of lactic in rat serum was developed by reversed-phase high performance liquid chromatography(RP-HPLC) with ultraviolet detector.Protein in serum samples was precipitated by perchloric acid solution,and Eclipse plus C18 column was used with 5% methanol-water solution containing 0.2% phosphoric acid as the mobile phase at a flow rate of 0.8mL·min-1,and detection wavelength was 210nm,quantified by external standard method.Lactic had good linear in the range of 4.88×10-3—5g·L-1(r=0.9999),and the detection limit was 1.5×10-3g·L-1.The method is fast,sensitive with good precision.

Key concepts: Chromatography, Phosphoric acid, Chemistry, Perchloric acid, Detection limit, High-performance liquid chromatography, Lactic acid, Linear range

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