Determination of Lactic Acid Content in Subcutaneous Micro-dialysis Fluid of Tumor-burdened Mice by HPLC

Objective: To establish a method for the determination of lactic acid content in subcutaneous micro-dialysis fluid of hepatoma-bearing mice by micro-dialysis combined with HPLC. Method: Micro-dialysis system was adopted to collect subcutaneous micro-dialysis fluid of normal mice and tumor-burdened mice for determining the content of lactic acid in subcutaneous micro-dialysis fluid by HPLC. The ZORBAX SB-Aq column( 4. 6 mm × 150 mm,5 μm) was used with the mobile phase of methanol-0. 1% phosphoric acid solution( 5∶ 95)at a flow rate of 1. 0 m L·min-1,the detection wavelength was 210 nm,and the column temperature was set at 30℃. Result: The linearity ranged between 20. 39-611. 6 mg·L-1with the average recovery rate of 97. 41% and RSD of 4. 1%. Conclusion: This method is simple,reliable,rapid and highly sensitive,it can accurately detect lactic acid content in dialyzate and provide a new way to detect lactic acid.