Preparation and identification of amoxicillin artificial antigen by EDC method.

To synthesize the artificial antigen of AMO and obtain its mouse polyclonal antiserum,the immunogen AMO-BSA and coating antigen AMO-OVA were synthesized using EDC method and identified by ultraviolet scanning and SDS-PAGE.BALB/c mice were immunized with the synthesized antigens and the polyclonal antiserum was determined by indirect and blocking ELISA.The results showed that after conjugation,the ultraviolet absorption peak of AMO-BSA appeared at 276 nm,indicating that certain displacement occurred comparing with the ultraviolet absorption peaks of both AMO and BSA.The electropharetic mobility of BSA was observed bigger than that of AMO-BSA.Indirect ELISA showed that the antiserum titres of all the three immunized BALB/c mice were above 1×10-4.With the IC50 of 573.75 ng/mL,No.1 mouse polyclonal antiserum showed the best sensitivity.In this study,AMO-BSA and AMO-OVA were successfully synthesized and high sensitive polyclonal antiserums against AMO were prepared,which provides a basis for the preparation of monoclonal antibodies against AMO.