Production of Multiclone Antibody of PFLX

To produce multiclone antibody of PFLX.PFLX were coupled with bovine serum albumin(BSA) and ovalbumin(OVA) by using N-hydroxysuccinimide actived ester method to prepare the immune antigen PFLX-BSA and detection antigen PFLX-OVA.The mice were immunized with PFLX-BSA to obtain the polyclonal antibody with high titer.The ultraviolet spectrum of immune antigen PFLX-BSA had the stacking characteristics of medicament and protein and its maximum absorption peak was 277.5 nm,which indicated that vector protein BSA was successfully coupled with PFLX and it could be used in animal immunization.The maximun absorption peak of detection antigen PFLX-OVA was 275nm,indicating vector protein OVA was successfully coupled with PFLX and it could taken as detection antigen for coating.The results of indirect ELISA detection showed that the antiserum titers of mice were higher and reached over 1∶32 000,which indicated that the specific antibody was produced.The polyclonal antibody against PFLX was successfully prepared,which further proved that PFLX were successfully coupled.