Effect of ischemic preconditioning on NOS and L-selectin expression in rat renal ischemic reperfusion

Objective:To observe the change of NO,NOS and L-selectin expression in rat renal ischemic reperfusion injury after renal ischemic preconditioning and investigate the effect of ischemic preconditioning on renal ischemic reperfusion injury.Methods: Thirty SD rats were randomly divided into three groups: sham-operated group,ischemic-reperfusion group,ischemic preconditioning group.The renal underwent 60-minute ischemic and 2-hour reperfusion.The level of Superoxide Dismutase(SOD),Malondialdehyde(MDA),NO,NOS of kidney,blood in each group were examined,L-selectin expression in kidney was tested with immunohistochemichemistry. Results: SOD: The values in ischemic-preconditioning group were significantly higher than that in ischemic-reperfusion group(P0.01),MDA: The values in ischemic-preconditioning group were significantly lower than that in ischemic-reperfusion group(P0.01),NOS,NO:The values in ischemic-preconditioning group were significantly higher than that in ischemic-reperfusion group.The expression of L-selectin in ischemic-preconditioning group was lower than that in ischemic-reperfusion group(P0.05). Conclusion: Ischemic preconditioning could protect kidney from ischemic reperfusion injury,the mechanism might be that it increased NOS in kidneys and blood,so that the expression of L-selection was inhibited,with improving the SOD′s activity and reducing the levels of MDA in kidney and blood.