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[Determination of cinnamaldehyde in Guizhi by HPLC].

J P Luo, Z S Zhang, Cui Tian, X M Li

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Abstract

OBJECTIVE: To establish a HPLC method for the determination of cinnamaldehyde in Guizhi. METHOD: Cinnamaldehyde was separated by a Kromasil C18 column (4.6 mm x 200 mm) with a mobile phase of acetonitrile-0.1% phosphoric acid solution(48:52) and an UV detection wavelength of 285 nm. RESULT: The calibration curve was linear in the range of 0.004-0.02 microliter.ml-1, with a correlation coefficient of 0.9996. The average recovery was 100.7%(n = 5), and RSD 0.3%(n = 6). CONCLUSION: The method is simple and accurate.

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What this paper is about

OBJECTIVE: To establish a HPLC method for the determination of cinnamaldehyde in Guizhi. METHOD: Cinnamaldehyde was separated by a Kromasil C18 column (4.6 mm x 200 mm) with a mobile phase of acetonitrile-0.1% phosphoric acid solution(48:52) and an UV detection wavelength of 285 nm. RESULT: The calibration curve was linear in the range of 0.004-0.02 microliter.ml-1, with a correlation coefficient of 0.9996. The average recovery was 100.7%(n = 5), and RSD 0.3%(n = 6). CONCLUSION: The method is simple and accurate.

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Available abstract

OBJECTIVE: To establish a HPLC method for the determination of cinnamaldehyde in Guizhi. METHOD: Cinnamaldehyde was separated by a Kromasil C18 column (4.6 mm x 200 mm) with a mobile phase of acetonitrile-0.1% phosphoric acid solution(48:52) and an UV detection wavelength of 285 nm. RESULT: The calibration curve was linear in the range of 0.004-0.02 microliter.ml-1, with a correlation coefficient of 0.9996. The average recovery was 100.7%(n = 5), and RSD 0.3%(n = 6). CONCLUSION: The method is simple and accurate.

Key concepts: Cinnamaldehyde, Calibration curve, Chromatography, Phosphoric acid, High-performance liquid chromatography, Correlation coefficient, Chemistry, Linear correlation

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