Determination of Three Flavonoids in Populus Canadensis Moench. Leaves

OBJECTIVE To establish an analytical method for simultaneous determination of quercetin, galangin and chrysin in Populus canadensis Moench. leaves. METHODS The determination was performed on Agilent TC C18 column(4.6 mm×250 mm, 5 μm) with the mobile phase consists of methanol-0.1% formic acid at a flow rate of 1.0 mL·min–1. The following gradient elution was used: the content of methanol was 25% to 55% from 0 to 5 min, 55% to 65% from 5 to 25 min and 65% to 75% from 25 to 35 min. The detection wavelength was set at 264 nm, and the column temperature was 30 ℃. RESULTS This method had a good separation performance for quercetin, galangin and chrysin in poplar leaves, and the linear range were 3.7-120, 2.5-80 and 3.125-100 μg·mL-1, respectively; the average recovery rates were 99%, 100.1% and 101.4%, respectively. CONCLUSION This method is accurate, reliable with good stability and repeatability which provides the evidence for quality evaluation for poplar leaves.