2008•Xiandai shengwu yixue jinzhanRequires access

Application of PEG/(NH_4)_2SO_4 Aqueous Two-phase System to Extraction of Total Flavone of Populus Canadensis Moench.Leaves

Shi Hui

Abstract

Objective:To find an effective method for extraction and purification of total fiavone of Populus Canadensis Moench. leaves.Methods:In the experiment,extraction and content determination of flavone of Populus Canadensis Moench.leaves were carried out simultaneously by using aqueous two-phase extraction partitioning and UV-spectrophotometry method.Results:This aqueous two-phase system was prepared by dissolving suitable amounts of polyethylene glycol (PEG)and (NH_4)_2SO_4 in aqueous solution.Flavone of Populus Canadensis Moench.leaves were recovered with optimum high yield and high concentration in the upper polymer-rich phase when 25% PEG 400 and 12%(NH_4)_2SO_4 were applied.The optimum extraction conditions were pH value 9,NaCl3 %,temperature 25℃and leaching solution volume 3 mL.The precision (RSD)was 0.28% (n=5).Conclusion:The method is proved to be good in repro- ducibility,simple and convenient in operation,and it is effective to extract flavone.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Polyethylene glycol, Extraction (chemistry), PEG ratio, Chemistry, Aqueous solution, Chromatography, Aqueous two-phase system, Yield (engineering)