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2008•The Chinese Journal of Modern Applied PharmacyRequires access

Orthogonal Test for Optimizing the Extracting Method of Total Flavonoids from Radix Tetrastigmae

Yingying Hu

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Abstract

OBJECTIVE To optimize extraction technology of the total flavonoids from Radix Tetrastigmae.METHODS In the method of one-way and orthogonal test, the ethanol concentration and amount, extraction time and number of extractions were studied with the total flavonoids from Radix Tetrastigmae as the target of the test. RESULTS The ethanol concentration had great impact on the target.The order of the other 3 factors that affected the flavonoid extractions was number of extractions﹥extraction time﹥amount of ethanol. CONCLUSION The parameters of the best extraction technology are as follows: 50% ethanol, 15 times the weight of the dried material, refluxing and extracting 2 h for 3 times.

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What this paper is about

OBJECTIVE To optimize extraction technology of the total flavonoids from Radix Tetrastigmae.METHODS In the method of one-way and orthogonal test, the ethanol concentration and amount, extraction time and number of extractions were studied with the total flavonoids from Radix Tetrastigmae as the target of the test. RESULTS The ethanol concentration had great impact on the target.The order of the other 3 factors that affected the flavonoid extractions was number of extractions﹥extraction time﹥amount of ethanol. CONCLUSION The parameters of the best extraction technology are as follows: 50% ethanol, 15 times the weight of the dried material, refluxing and extracting 2 h for 3 times.

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Available abstract

OBJECTIVE To optimize extraction technology of the total flavonoids from Radix Tetrastigmae.METHODS In the method of one-way and orthogonal test, the ethanol concentration and amount, extraction time and number of extractions were studied with the total flavonoids from Radix Tetrastigmae as the target of the test. RESULTS The ethanol concentration had great impact on the target.The order of the other 3 factors that affected the flavonoid extractions was number of extractions﹥extraction time﹥amount of ethanol. CONCLUSION The parameters of the best extraction technology are as follows: 50% ethanol, 15 times the weight of the dried material, refluxing and extracting 2 h for 3 times.

Key concepts: Radix (gastropod), Extraction (chemistry), Chromatography, Flavonoid, Ethanol, Chemistry, Mathematics, Botany

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