Study on the Process for Extraction of Total Flavonoids from Radix of Pueraria

Objective To optimize the extracting process for total flavonoids from radix Puerariae. Methods Single factor and orthogonal design were respectively utilized to optimize extracting process of radix Puerariae. Concentration of total flavonoids was measured by the UV spectrophotometer and concentration of puerarin was measured by HPLC. The content of puerarin and the total flavonoids were used as indexes to evaluate the optimal extracting conditions. Results The results were showed that the optimum extraction process were as follows 30% alcohol as solvent 9 times of alcohol amount 3 times of extraction and 1 h per time. Conclusion This process was simple and feasible with good effect of extraction which can meet with the industrial requirements.