Distribution and drug resistance of clinical isolates of major gram-positive cocci

OBJECTIVE To understand the distribution of clinical isolates of major gram-positive cocci in Jiangsu Shengze Hospital in 2010-2012 and analyze the change of drug resistance so as to provide guidance for clinical use of antibiotics.METHODS From 2010 through 2012,the related data of the gram-positive cocci isolated from various specimens obtained from 418 hospitalized patients were retrospectively analyzed,then the bacterial identification was performed with the use of API system,the drug susceptibility testing was conducted by means of disk diffusion method,and the data were analyzed by using WHONET5.6software.RESULTS A total of 418 strains of gram-positive cocci have been isolated from 2010 to 2012,among which the Staphylococcus spp accounted for79.9%,the Enterococcus spp13.4%,the Streptococcus spp6.7%.The isolation rate of the methicillin-resistant Staphylococcus aureus(MRSA)was 68.6%in 2010,52.3%in 2011,64.1%in 2012;the isolation rate of the methicillin-resistant coagulase-negative Staphylococcus(MRCNS)was 84.1%in 2010,75.4%in 2011,74.3%in2012.The drug resistance rate of the MRSA was significantly higher than that of the methicillin-susceptible Staphylococcus aureus(MSSA);the MRCNS was highly susceptible to piperacillin-tazobactam and amikacin;no strain resistant to linezolid,vancomycin,or teicoplanin has been detected;the drug resistance rates of the Enterococcus sppto penicillin,erythromycin,levofloxacin,and ampicillin were more than 50.0%;the drug resistance rates of the Streptococcus sppto penicillin and erythromycin were more than 70.0%;no strain resistant to vancomycin or teicoplanin has been detected.CONCLUSIONThe drug resistance rates of the isolated gram-positive cocci show an upward trend.It is crucial for the reasonable clinical use of antibiotics and delay of emergence of drug-resistant strains to strengthen the surveillance of drug resistance of the pathogens causing nosocomial infections and understand the change of drug resistance in a timely manner.