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2011•Unpublished venueRequires access

Determination of paeoniflorin in Danggui Huoxue capsules with HPLC

Deng Man-jing

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Abstract

Objective To establish a high performance liquid chromatography(HPLC) method for the determination of paeoniflorin in Danggui Huoxue capsule.Methods The separation of paeoniflorin was performed on Diamonsil C18 column with a detection wavelength of 230 nm.The mobile phase was composed of acetonitrile-0.1% phosphoric acid solution(14:86).Result The linear range of paeoniflorin was 0.142～1.42 mg(r=0.999 8).The average recovery was 97.28% and the RSD was 1.60%.Conclusion The method is simple,accurate with good reproducibility and can be used for the quality control of Dangguihuoxue Capsule.

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What this paper is about

Objective To establish a high performance liquid chromatography(HPLC) method for the determination of paeoniflorin in Danggui Huoxue capsule.Methods The separation of paeoniflorin was performed on Diamonsil C18 column with a detection wavelength of 230 nm.The mobile phase was composed of acetonitrile-0.1% phosphoric acid solution(14:86).Result The linear range of paeoniflorin was 0.142～1.42 mg(r=0.999 8).The average recovery was 97.28% and the RSD was 1.60%.Conclusion The method is simple,accurate with good reproducibility and can be used for the quality control of Dangguihuoxue Capsule.

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Available abstract

Objective To establish a high performance liquid chromatography(HPLC) method for the determination of paeoniflorin in Danggui Huoxue capsule.Methods The separation of paeoniflorin was performed on Diamonsil C18 column with a detection wavelength of 230 nm.The mobile phase was composed of acetonitrile-0.1% phosphoric acid solution(14:86).Result The linear range of paeoniflorin was 0.142～1.42 mg(r=0.999 8).The average recovery was 97.28% and the RSD was 1.60%.Conclusion The method is simple,accurate with good reproducibility and can be used for the quality control of Dangguihuoxue Capsule.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Phosphoric acid, Capsule, Linear range, Chemistry, Reproducibility

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