ScholarLens Back to search
2006•Journal of Henan University of TechnologyRequires access

GENOMIC DNA EXTRACTION METHOD OF AQUILARIA SINENSIS(LOUR.)GILG

Zhao Xuan, Zhao Shu-jin

Open publisher page 0 citations

Abstract

A modified CTAB method of genomic DNA extraction of Aquilaria sinensis(Lour.)Gilg was established based on the classic CTAB method.The comparison of modified CTAB method and Kit method showed that the quality of the genomic DNA extracted by the modified CTAB method was better than that of Kit method.The OD-(260)/OD-(280) ratio of the genomic DNA extracted with modified CTAB method was between 1.8 and 2.0 and the DNA yield was about 172 μg/100 mg dry leaf.The DNA extracted with the modified CTAB method could be digested completely by restriction endonuclease to be used for downstream studies.Conclusion: The modified CTAB method was suitable for the extraction of the genomic DNA of Aquilaria sinensis(Lour.)Gilg..

About this research paper

What this paper is about

A modified CTAB method of genomic DNA extraction of Aquilaria sinensis(Lour.)Gilg was established based on the classic CTAB method.The comparison of modified CTAB method and Kit method showed that the quality of the genomic DNA extracted by the modified CTAB method was better than that of Kit method.The OD-(260)/OD-(280) ratio of the genomic DNA extracted with modified CTAB method was between 1.8 and 2.0 and the DNA yield was about 172 μg/100 mg dry leaf.The DNA extracted with the modified CTAB method could be digested completely by restriction endonuclease to be used for downstream studies.Conclusion: The modified CTAB method was suitable for the extraction of the genomic DNA of Aquilaria sinensis(Lour.)Gilg..

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A modified CTAB method of genomic DNA extraction of Aquilaria sinensis(Lour.)Gilg was established based on the classic CTAB method.The comparison of modified CTAB method and Kit method showed that the quality of the genomic DNA extracted by the modified CTAB method was better than that of Kit method.The OD-(260)/OD-(280) ratio of the genomic DNA extracted with modified CTAB method was between 1.8 and 2.0 and the DNA yield was about 172 μg/100 mg dry leaf.The DNA extracted with the modified CTAB method could be digested completely by restriction endonuclease to be used for downstream studies.Conclusion: The modified CTAB method was suitable for the extraction of the genomic DNA of Aquilaria sinensis(Lour.)Gilg..

Key concepts: genomic DNA, Restriction enzyme, DNA extraction, Extraction (chemistry), Biology, DNA, Chemistry, Chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
GENOMIC DNA EXTRACTION METHOD OF AQUILARIA SINENSIS(LOUR.)GILG — Research Paper | ScholarLens