Comparison on Genomic DNA Extracted from the Leaves of Chinese Chestnut by Two Methods

New leaves of nine Castanea mollissima Blume varieties from Luotian country Hubei province were used as experimental materials and genomic DNA extraction methods of plant genomic DNA Kit and modified CTAB were used and compared.The results showed that the method of plant genomic DNA Kit was poor and was difficult to obtain clear bands by agarose gel electrophoresis.The ratio A260/A280 of extracted DNA was 1.11~1.49 and the yield was 24.50~59.25μg/g.However,PVP and 2-mercaptoethanol were added to CTAB buffer in order to inhibit oxidation of polyphenol,and elution for many times were used to remove polysaccharide could be inhibited and most of polysaccharides in leaves could be removed efficiently.The ratio A260/A280 of DNA extracted by modified CTAB ranged from 1.80~1.90,and the yield was 60.50~102.00μg/g.The result of Castanea mollissima Blume molecular marker also showed that the modified CTAB method was suitable for extracting high qualified DNA fromCastanea mollissima Blume.