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[Determination of jujubaside A in semen ziziphi spinosae by RP-HPLC].

Li Yj, Che Zt, Bi Ks, Xu Zm, Li M

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Abstract

OBJECTIVE: To develop a new method for the determination of Jujubaside A in Semen Ziziphi Spinosae. METHOD: A RP-HPLC method was set up, using Hypersil-18 column, acetonitrile: water(30:70) as mobile phase with a flow rate of 0.8 ml.min-1. RESULT: The calibration curve was linear in the range of 0.076-0.456 mg. The average recovery was 93.5%. CONCLUSION: The method is appropriate for the determination of Jujubaside A.

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What this paper is about

OBJECTIVE: To develop a new method for the determination of Jujubaside A in Semen Ziziphi Spinosae. METHOD: A RP-HPLC method was set up, using Hypersil-18 column, acetonitrile: water(30:70) as mobile phase with a flow rate of 0.8 ml.min-1. RESULT: The calibration curve was linear in the range of 0.076-0.456 mg. The average recovery was 93.5%. CONCLUSION: The method is appropriate for the determination of Jujubaside A.

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Available abstract

OBJECTIVE: To develop a new method for the determination of Jujubaside A in Semen Ziziphi Spinosae. METHOD: A RP-HPLC method was set up, using Hypersil-18 column, acetonitrile: water(30:70) as mobile phase with a flow rate of 0.8 ml.min-1. RESULT: The calibration curve was linear in the range of 0.076-0.456 mg. The average recovery was 93.5%. CONCLUSION: The method is appropriate for the determination of Jujubaside A.

Key concepts: Chromatography, High-performance liquid chromatography, Calibration curve, Semen, Chemistry, Linear relationship, Mathematics, Medicine

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