Studies on Qualitative and Quantitative Methods for Herba Rabdosiae Serrae

Objective To optimize the qualitative and quantitative methods for Herba Rabdosiae Serrae,thus to improve its quality standard. Methods Thin layer chromatography(TLC)was performed for identifying rosmarinci acid,oleanolic acid and rutin. Rosmarinci acid was determined by high performance liquid chromatography(HPLC). The HPLC was conduced with octadecylsilane chemically bonded silica as loading agent,acetonitrile- 0.1 % phosphoric acid solution(30∶70)as mobile phase,and the detection wavelength was 330 nm,flow rate was 1.0 mL·min-1and column temperature was 25 ℃. The number of theoretical plates for rosmarinci acid was not less than 4000. Results The TLC spots were clear and can be well separated,indicating that the method had good ruggedness. The HPLC method was simple and specific. The calibration curve showed good linear relationship in the range of 1.2 μg~12 μg(r=0.9999). The average recovery was 98.37 % and RSD was 1.20 %(n=6). Conclusion The developed qualitative and quantitative methods can be used for the qualitycontrol ofHerba Rabdosiae Serrae.