ScholarLens Back to search
2010•Journal of Guangdong Medical CollegeRequires access

Effect of arsenic trioxide on apoptosis and VEGF expression of human Hela cells

Qiuwei Li

Open publisher page 0 citations

Abstract

Objective To study the effect of arsenic trioxide (As2O3) on apoptosis and VEGF expression of human HeLa cells. Methods HeLa cells were treated with As2O3 at various concentrations for different time points, and their growth inhibi-tion, apoptosis and VEGF expression were determined by MTT, flow cytometry and Western blotting, respectively. Results As2O3 pretreatment induced the growth inhibition and apoptosis of HeLa cells in a dose-and time-dependent manner (P0.01), and downregulation of VEGF expression. Conclusion As2O3 can in vitro inhibit cell proliferation and VEGF expression of human HeLa cells.

About this research paper

What this paper is about

Objective To study the effect of arsenic trioxide (As2O3) on apoptosis and VEGF expression of human HeLa cells. Methods HeLa cells were treated with As2O3 at various concentrations for different time points, and their growth inhibi-tion, apoptosis and VEGF expression were determined by MTT, flow cytometry and Western blotting, respectively. Results As2O3 pretreatment induced the growth inhibition and apoptosis of HeLa cells in a dose-and time-dependent manner (P0.01), and downregulation of VEGF expression. Conclusion As2O3 can in vitro inhibit cell proliferation and VEGF expression of human HeLa cells.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the effect of arsenic trioxide (As2O3) on apoptosis and VEGF expression of human HeLa cells. Methods HeLa cells were treated with As2O3 at various concentrations for different time points, and their growth inhibi-tion, apoptosis and VEGF expression were determined by MTT, flow cytometry and Western blotting, respectively. Results As2O3 pretreatment induced the growth inhibition and apoptosis of HeLa cells in a dose-and time-dependent manner (P0.01), and downregulation of VEGF expression. Conclusion As2O3 can in vitro inhibit cell proliferation and VEGF expression of human HeLa cells.

Key concepts: HeLa, Arsenic trioxide, Apoptosis, Flow cytometry, Chemistry, MTT assay, In vitro, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of arsenic trioxide on apoptosis and VEGF expression of human Hela cells — Research Paper | ScholarLens