Establishment of High Frequency Regeneration System of Medicago sativa

Taking the cotyledons of Medicago sativa‘Gannong No.l'as explants,the effects of different induction media and different concentrations of hormone on influence of callus induction,differentiation and rooting were investigated.The results showed that NSH+2,4-D 4mg/L+6-BA 0.4mg/L+3%sucrose+0.7%agar was the optimal medium for callus induction;MS+2%sucrose+0.7%agar was the optimal media for differentiation;MS+NAA 0.2mg/L+1% sucrose+0.7%agar was the optimal medium for rooting.The regenerative plant could survive after transplanting into the suitable substrate consisted of nutritive soil∶peat∶vermiculite=1∶2∶1.This study built an stable high frequency regeneration system for alfalfa,and laid a foundation for the transgenic receptor.