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Determination of Content of Cinnamicaldehyde in Shengui Capsule by HPLC

Ming Li

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Abstract

Objective To establish the quantitative method of cinnamaldehyde in Shengui capsule by high performance liquid chromatography.Methods This assay was performed on Inerstil ODS-3 C18(4.6 mm×250 mm,5 μm)column.The mobile phase of acetonitrile-water(32∶68) in isocratic elution at a flow rate of 1.0 ml/min.And the detection wavelength was 290 nm.The column temperature was 40 ℃.The injection volume of 10 μl.Results cinnamicaldehyde had good linearity within the range of 0.017 8~0.355 8 μg.And their mean recovery rates were 100.2%(n=6).Conclusion The method is convenient,sensitive,reproducible,specific,and can be used for the quality control of the product.

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What this paper is about

Objective To establish the quantitative method of cinnamaldehyde in Shengui capsule by high performance liquid chromatography.Methods This assay was performed on Inerstil ODS-3 C18(4.6 mm×250 mm,5 μm)column.The mobile phase of acetonitrile-water(32∶68) in isocratic elution at a flow rate of 1.0 ml/min.And the detection wavelength was 290 nm.The column temperature was 40 ℃.The injection volume of 10 μl.Results cinnamicaldehyde had good linearity within the range of 0.017 8~0.355 8 μg.And their mean recovery rates were 100.2%(n=6).Conclusion The method is convenient,sensitive,reproducible,specific,and can be used for the quality control of the product.

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Available abstract

Objective To establish the quantitative method of cinnamaldehyde in Shengui capsule by high performance liquid chromatography.Methods This assay was performed on Inerstil ODS-3 C18(4.6 mm×250 mm,5 μm)column.The mobile phase of acetonitrile-water(32∶68) in isocratic elution at a flow rate of 1.0 ml/min.And the detection wavelength was 290 nm.The column temperature was 40 ℃.The injection volume of 10 μl.Results cinnamicaldehyde had good linearity within the range of 0.017 8~0.355 8 μg.And their mean recovery rates were 100.2%(n=6).Conclusion The method is convenient,sensitive,reproducible,specific,and can be used for the quality control of the product.

Key concepts: Chromatography, Capsule, Cinnamaldehyde, High-performance liquid chromatography, Volumetric flow rate, Acetonitrile, Chemistry, Content determination

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