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2004•Unpublished venueRequires access

Quantitative determination and content uniformity of niferex capsule by HPLC

Wang Jia-yi

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Abstract

Objective A method to determinate the ferric content of the niferex capsule in the acidic medium by HPLC was established. Methods HPLC Nuoleosil ODS Column(250 mm×4.6 mm,10 μm),the mobile phase constisted of Acetonitnle-Teralammonium hydroxide solution (6:94),flow rate 1.5 mL/min,Detection was done at 300nm. Results The calibration curves were linear in the range of 19.77～98.89 μg/mL. The average recoveries(n=9) were 99.4% (RSD=1.5%). Conclusion This method is accurate ,good reproducibility and is very suitable for Niferex Capsule.

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What this paper is about

Objective A method to determinate the ferric content of the niferex capsule in the acidic medium by HPLC was established. Methods HPLC Nuoleosil ODS Column(250 mm×4.6 mm,10 μm),the mobile phase constisted of Acetonitnle-Teralammonium hydroxide solution (6:94),flow rate 1.5 mL/min,Detection was done at 300nm. Results The calibration curves were linear in the range of 19.77～98.89 μg/mL. The average recoveries(n=9) were 99.4% (RSD=1.5%). Conclusion This method is accurate ,good reproducibility and is very suitable for Niferex Capsule.

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Available abstract

Objective A method to determinate the ferric content of the niferex capsule in the acidic medium by HPLC was established. Methods HPLC Nuoleosil ODS Column(250 mm×4.6 mm,10 μm),the mobile phase constisted of Acetonitnle-Teralammonium hydroxide solution (6:94),flow rate 1.5 mL/min,Detection was done at 300nm. Results The calibration curves were linear in the range of 19.77～98.89 μg/mL. The average recoveries(n=9) were 99.4% (RSD=1.5%). Conclusion This method is accurate ,good reproducibility and is very suitable for Niferex Capsule.

Key concepts: Chromatography, Capsule, Reproducibility, Calibration curve, High-performance liquid chromatography, Chemistry, Content determination, Linear range

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