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2008•China Medical HeraldRequires access

HPLC determination for Yaoxitong capsule

Jing Liu

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Abstract

Objective:To develop a method for detemination for Yaoxitong Capsule by HPLC. Methods:The Agilent C18 (4.6 mm×150 mm,5 μm) was used, Methanol-Water- Acetic acid(20∶80∶0.5) as the mobile phase, the flow rate was 1.0 ml/min, the detection wavelength was 249 nm. The injected quantity of 20 μl Results:The linearity of peak area was good when the injected quantity of Granisetion was in the range of 20～200 μg/ml(r=0.999 96,n=5), The recovery of this method was 99.2%,RSD:0.52%. Conclusion:This method is convenient,accurate and the results of experiment satisfactory.

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What this paper is about

Objective:To develop a method for detemination for Yaoxitong Capsule by HPLC. Methods:The Agilent C18 (4.6 mm×150 mm,5 μm) was used, Methanol-Water- Acetic acid(20∶80∶0.5) as the mobile phase, the flow rate was 1.0 ml/min, the detection wavelength was 249 nm. The injected quantity of 20 μl Results:The linearity of peak area was good when the injected quantity of Granisetion was in the range of 20～200 μg/ml(r=0.999 96,n=5), The recovery of this method was 99.2%,RSD:0.52%. Conclusion:This method is convenient,accurate and the results of experiment satisfactory.

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Available abstract

Objective:To develop a method for detemination for Yaoxitong Capsule by HPLC. Methods:The Agilent C18 (4.6 mm×150 mm,5 μm) was used, Methanol-Water- Acetic acid(20∶80∶0.5) as the mobile phase, the flow rate was 1.0 ml/min, the detection wavelength was 249 nm. The injected quantity of 20 μl Results:The linearity of peak area was good when the injected quantity of Granisetion was in the range of 20～200 μg/ml(r=0.999 96,n=5), The recovery of this method was 99.2%,RSD:0.52%. Conclusion:This method is convenient,accurate and the results of experiment satisfactory.

Key concepts: Medicine, Capsule, Chromatography, High-performance liquid chromatography, Acetic acid, Linearity, Biomedical engineering, Biology

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