Gene Polymorphism in Promoter of Interleukin-6 between Hui and Han Ethnic Group of Ningxia

Objective To explore the frequencies of allele and genotype distribution of interleukin6(IL-6) gene promoter-572G/C,-597G/A and-174G/C polymorphisms in the Hui and Han people of Ningxia Province and to analyze the distributions of IL-6 polymorphism among different places and ethnic groups.Methods The gene IL-6-572G/C,-597G/A and-174G/C polymorphisms was examined by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) methods in 210 hui and 308 han healthy adults.Results IL-6-572,-597,-174 genotype frequencies were-572(CC:55.71%;CG:34.76%;GG:9.52%;C:73.10%;G:26.90%),-597(GG:94.29%;GA:5.71%;AA:0%;G:97.14%;A:2.86)and-174(GG:96.67%;GC:3.23%;CC:0%;G:98.33%;C:1.67%) in Ningxia Hui ethnic group,respectively.IL-6-572,-597,-174 genotypes frequencies were-572(CC:52.92%;CG:35.71%;GG:11.36%;C:70.78%;G:29.22%),-597(GG:92.86%;GA:7.14%;AA:0%;G:96.13%;A:3.57),-174(GG:97.08%;GC:2.92%;CC:0%;G:98.54%;C:1.46%) in Ningxia Han ethnic group,respectively.The Phenotype frequencies and allele gene frequencies of IL-6 gene showed no significant difference between the Hui and Han ethnicity in Ningxia(P0.05).The Phenotype frequency and allele gene frequency of IL-6 gene showed significant difference between the females and males in Ningxia.(P0.05).Conclusion The three SNPs of IL-6 genes distribution among the Hui and Han ethnicity of Ningxia have no significant difference.