Study on promoter of TGF-β1 gene polymorphism in Ningxia Hui and Han ethnic

Objective To study the frequencies of allele and genotype distribution of transforming growth factor-β1(TGF-β1) gene promoter-800(G/A) and SNP-509(C/T) polymorphisms in the Hui and Han people in Ningxia,and to analyze the distributions of TGF-β1 gene polymorphism among different places and race.Methods The gene TGF-β1-800(G/A) and SNP-509(C/T) polymorphisms were examined by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) methods in 210 Hui and 308 Han healthy adults.Results TGF-β1-800(G/A) and SNP-509(C/T) genotypes frequencies were-800(GG: 97.62%;GA: 2.38%;AA: 0%;G:98.81%;A: 1.19%) and-509(CC: 21.90%;CT: 56.67%;TT: 21.43%;C: 50.24%;T: 49.76%) in Ningxia Hui ethnic,respectively.TGF-β1-800,-509 genotypes frequencies were-800(GG: 99.03%;GA: 0.97%;AA: 0%;G: 99.52%;A: 0.48%);-509(CC: 27.27%;CT: 50.97%;TT: 21.75%;C: 52.76%;T: 47.24%) in Ningxia Han ethnic,respectively.The phenotype frequency and allele gene frequency of TGF-β1 gene were not significantly different between the Hui and Han ethnic in Ningxia(P0.05).The phenotype frequency and allele gene frequency of TGF-β1 gene were not significantly different between the female and male people in Ningxia(P0.05).Conclusion The two SNPs of TGF-β1 genes distribution is not significantly different in the Hui and Han ethnic of Ningxia.