2013•Guoshu xuebaoRequires access
Effect of trichoderma viride fermentation broth on Colletotrichum gloeosporioides defensive enzyme activities
Shuyu Liu, Yu Xin, Chen Fahe, Yang Pengbin, Li Liu, Huang Xiao-min
Open publisher page 2 citations
Abstract
【Objective】The mechanism of Trichoderma viride fermentation against Colletotrichum gloeosporioides Penz defensive enzyme’s activity were explored.【Method】C.gloeosporioides Penz was treated with T.viride fermentation broth for 0.0 to 30.0 h which reached MIC50(1% v/v),then CAT,POD,PPO,SOD,PAL and dehydrogenase activities of C.gloeosporioides Penz were determined.【Result】 T.viride fermentation broth restrained the activity of C.gloeosporioides Penz’s defensive enzyme,which led to the damage of cell membrane protective system,leak of intracellular substances,and finally suppressed or killed C.gloeosporioides Penz.The activity of CAT,POD,PPO,PAL,SOD and dehydrogenase in C.gloeosporioides Penz,treated with T.viride fermentation for 30 h were 52.54%,23.62%,58%,6.38%,6.52% and 44.74% lower than control,respectively.【Conclusion】T.viride fermentation broth could significantly inhibit defensive enzyme of C.gloeosporioides Penz,and restrain the growth of C.gloeosporioides Penz.