Effect of Trichoderma uiride fermentation broth on Colletotrichum gloeosporioides growth inhibition and cell damage

【Objective】The mechanism of Trichoderma viride fermentation broth against Colletotrichum gloeosporioides Penz was studied.【Method】C.gloeosporioides Penz,was treated with T.viride fermentation broth,then the hypha growth,spore germination,absorption of phosphoru and membrane permeability of C.gloeosporioides Penz was determined.【Result】The hypha treated with T.viride fermentation broth withered and deformed seriously,and the spore germination in the test group was 86.40% lower than in the control group.Besides,the absorption of phosphorus was almost stopped,both nucleic acid and protein outflowed seriously after the application of T.viride fermentation broth;the contents of reducing sugar and soluble protein in test group were significantly lower than in control group.【Conclusion】T.viride fermentation broth could undermine the integrity of C.gloeosporioides Penz's cell,and suppress the growth of C.gloeosporioides Penz.